- Alessandro de Medici 1531-1537
- Cosimo I de Medici 1537-1569
- Cosimo I de Medici 1569-1574
- Francesco de Medici 1574-1587
- Ferdinando I de Medici 1587-1609
- Cosimo II de Medici 1609-1621
- Ferdinando II de Medici 1621-1670
- Cosimo III de Medici 1670-1723
- Gian Gastone de Medici 1723-1737
- Louis 1801-1803
- Charles Louis 1803-1807
Tuscany was annexed by France, 1807-1814
- Ferdinand III (restored) 1814-1824
- Leopold II[?] 1824-1848
- provisional republic 1848-1849
- Leopold II (restored) 1849-1859
- Ferdinand IV[?] 1859-1860
Tuscany annexed by Piedmont-Sardinia[?], 1860